Department of Nutrition and Food Engineering
Dr. Sheikh Mahatabuddin  Associate Professor
Name :
Dr. Sheikh Mahatabuddin
Employee ID
710002386
Designation
Associate Professor  
Department
Department of Nutrition and Food Engineering
Faculty
Faculty of Allied Health Science
Personal Webpage
https://faculty.daffodilvarsity.edu.bd/profile/nfe/mahatabuddin.html
E-mail
mahatabuddin.ged@diu.edu.bd
Phone
Cell-Phone
+8801842488337
Ph. D. : Doctor of Life Science, Graduate School of Life Science, Hokkaido University, Japan (2017) (MEXT Scholar)
M. S. : M. S. in Chemistry, Department of Chemistry, University of Dhaka (2009)
B. Sc. : B. Sc. Honors in Chemistry, Department of Chemistry, University of Dhaka, (2008)
Preparation of Future Faculty (PFF) : Training program to prepare graduate students as future leader in academia jointly organized by Hokkaido University, Japan and University of California, Berkeley. Completed with AA (Excellent) grade. (March, 2015)
RCRA Hazardous Waste Awareness Training for Generators : Training program organized by Henry Ford Health System University, MI, USA. Completed with 95% credit. (April, 2018)
Training on Breast Oncology : Training program organized by Henry Ford Cancer Institute, MI, USA. 5.5 non-physician (USA equivalence) credit hours was awarded (September, 2018)
IACUC Training for Laboratory Animal Handling : Training program organized by Henry Ford Health System University, MI, USA. Completed with 95% credit. (December, 2017)
General Safety and Hazard Communication Training : Training program organized by Tufts University, MA, USA. 0.5 non-physician (USA equivalence) credit hours was awarded (May, 2017)
Annual Laboratory Safety Training : Training program organized by Tufts University, MA, USA. Completed with 80% credit (June, 2017)
Biosafety in Research Laboratories (BRL) training : Training program organized by Tufts University, MA, USA. Completed with excellent credit (June, 2017)
Bloodborne Pathogen (BBP) : Training program organized by Tufts University, MA, USA. Completed with 80% credit (June, 2017)
  • Biochemistry and Molecular Biology
  • Advanced Analytical Techniques in Pharmaceutical Technology
  • Organic, Inorganic, Physical Pharmacy and Medicinal Chemistry
  • General Chemistry (for Engineering Undergrads)
  • Pharmaceutical Analysis and Quality Control
  • Cold Chain and Frozen Food Technology

Manuscripts:

5. Mahatabuddin S, Fukami D, Arai T, Nishimiya Y, Shimizu R, Shibazaki C, Kondo H, Adachi M, Tsuda S (2018) Polypentagonal ice-like water networks emerge solely in an activity-improved variant of ice-binding protein. Proc. Natl. Acad. Sci. U. S. A. 201800635 (https://doi.org/10.1073/pnas.1800635115).
4. Ghann W, Kang H, Uddin J, Gonawala SJ, Mahatabuddin S, Ali MM (2018) Dendrimer-based nanoparticle for dye sensitized solar cells with improved efficiency. Journal of Nanomedicine and Nanotechnology, 9(2), DOI:10.4172/2157-7439.1000496.
3. Mahatabuddin S, Hanada Y, Nishimiya Y, Miura A, Kondo H, Davies PL, Tsuda S (2017) Concentration-dependent oligomerization of an a-helical polypeptide makes it hyperactive. Scientific Reports 7, 42501. DOI:  10.1038/srep42501.
2.Mahatabuddin S, Nishimiya Y, Miura A, Kondo H and Tsuda S (2016) Critical ice shaping concentration: A new parameter to evaluate the activity of antifreeze proteins. Cryobiology and Cryotechnology, 62 (02), 95-103.
1. Arai T, J. Cheng, Mahatabuddin S, Kondo H and Tsuda S (2015), Observation of inhibitory effect of antifreeze protein on progressive freeze-concentration, Cryobiology and Cryotechnology, 61 (02), 121-124.

Book Chapter:

1. Mahatabuddin S and Tsuda S (2018) Applications of Antifreeze Proteins: Practical Use of the Quality Products from Japanese Fishes: Adaptation Mechanisms and Their Applications (Chapter 17), Survival Strategies in Extreme Cold and Desiccation: Adaptation Mechanisms and their Application, Iwaya-Inoue M., Sakurai M. and Uemura M. (Eds), 321-337, DOI: 10.1007/978-981-13-1244-1_17

Conferences, Symposia and Workshops:

15. Mahatabuddin S and Tsuda S: “Crystal structure substantiate the anchor clathrate water (ACW) mechanism for the moderate active antifreeze proteins” The 5th Conference of Bangladesh Crystallographic Association, Dhaka University, Dhaka Bangladesh; 25-26 January 2019 (Oral presentation)
14. Mahatabuddin S and Tsuda S: “Introduction of a new parameter critical ice shaping concentration (CISC) for evaluation of the activity of antifreeze proteins” The 4th International Life Science Symposium, Conference hall of Hokkaido University, Sapporo, Japan; 18 November/2016. (Oral presentation)
13. Rahman AT, Mahatabuddin S, Ohyama Y, Kondo H and Tsuda S:“Quantitative Methods to Evaluate Ice RecrystallizationKinetics of AFP Solutions” The 5th International Life Science Symposium, Conference hall of Hokkaido University, Sapporo, Japan; 28 October/2017. (Oral presentation)
12. Rahman AT, Mahatabuddin S, Ohyama Y, Kondo H and Tsuda S:“Quantitative Method to Evaluate Ice Recrystallization Kinetics of AFP Solutions” 26th Chemical Engineering Conference, Hokkaido University, Sapporo, Japan; 27 January/2017. (Oral presentation)
11. Fukami D, Mahatabuddin S, Kondo H, Miura A and Tsuda S: “Activity modification by mixing of antifreeze proteins” The 4th International Life Science Symposium, Conference hall of Hokkaido University, Sapporo, Japan; 18 November/2016. (Oral presentation)
10. Rahman AT, Mahatabuddin S, Ohyama Y, Kondo H and Tsuda S: “Quantitative methods to evaluate ice recrystallization inhibition rate using Ferret’s diameter” The 4th International Life Science Symposium, Conference hall of Hokkaido University, Sapporo, Japan; 18 November/2016. (Poster presentation)
9. Takeyama M, Mahatabuddin S, Miura A, Kondo H and Tsuda S: "Cell preservation mechanism of fish type III antifreeze protein using artificial lipid bilayer" The 89th Annual Meeting of the Japanese Biochemical Society, Sendai International Center, Tohoku University, Japan; 25-27 September/2016 (Oral and poster presentation)
8. Mahatabuddin S, Hanada Y, Miura A, Kondo H, and Tsuda S: Ice-accumulation ability of an alpha-helical peptide shows a significant concentration dependence. The 3rd International Life Science Symposium, Conference hall of Hokkaido University, Sapporo, Japan; 26 November/2015. (Oral presentation)
7. Mahatabuddin S, Ishihara K, Hanada Y, Miura A, Kondo H and Tsuda S: Expression and Characterization of a New Type I Antifreeze protein from a Japanese Fish. The 2nd International Life Science Symposium, Conference hall of Hokkaido University, Sapporo, Japan; 23 October/2014. (Oral presentation)
6. Mahatabuddin S, Ishihara K, Hanada Y, Miura A, Kondo H and Tsuda S: Functional Analysis of a New Type I Antifreeze Protein from Barfin plaice, Liposetta pinnifasciata. The 52nd Annual Meeting of Biophysical Society Japan (BSJ), Sapporo Convention center, Sapporo, Japan; 25-27 September/2014. (Poster presentation)
5. Mahatabuddin S, Ishihara K, Hanada Y, Miura A, Kondo H and Tsuda S: A 40-residue Type I Antifreeze Protein exhibited an extremely high thermal hysteresis activity. 2nd International Ice-Binding Protein Conference, Conference hall of Hokkaido University, Sapporo, Japan; 04-08 August/2014. (Poster and Oral presentation)
4. The 2nd Annual Henry Ford Cancer Institute Multi-Disciplinary Breast Oncology Symposium, Dearborn, MI, USA, September 28, 2018. (Participant)
3. Cell Press LabLinks meeting Small Molecule Metabolites as Signaling Molecules, Pfizer, 1 Portland Street, Cambridge, MA, USA; 11 September/2017. (Participant)
2. Workshop onPreparing Future Faculty (PFF): An introduction to teaching and writing for graduate students; Organized by: University of California Berkeley and Hokkaido University, Sapporo, Japan, March 16-20, 2015. (Participant)
1. The 1st International Life-Science Symposium (ILSS); Sapporo, Japan, March 6, 2013. (Participant)

Field of Specializatio:

1. Biophysics & Structural Biology
2. Ice Physics, Frozen Foods & Cryotechnology
3.  Cancer Biology and Cancer statistics
4. Biophysical and Chemical Kinetics
5. Protein Engineering

News Coverage of the Research Achievements:

1. Discovery of high function BpAFP;   
2. Discovery of high function BpAFP;     
3. How antifreeze proteins inhibit ice crystal growth
4. A water molecule network similar to ice has been discovered on the molecular surface of AFP

Skills:

Molecular Biology: RNA and DNA extraction, cDNA cloning, SDS-PAGE, Gel electrophoresis, rtPCR, qPCR, Reverse transcription, Western blotting, Cell survival assay, Gene expression assay, Yeast surface display, mammalian cell preservation, Serial Cloner, Genious.

Biophysics and Proteomics: Expression and purification of recombinant protein in E. coli, yeast, mammalian cell culture methods, Functional protein purification to technological application, Site-specific mutation, Isotopic labelling of proteins, Protein crystallization, Biochemical and biophysical characterization of protein, Affinity chromatography, Ion exchange chromatography, Size exclusion chromatography, PyMOL, HEX, EasyModeller, COOT.
Chemistry: Chemical kinetics, Chemical synthesis, TLC, HPLC, Column chromatography, Rotary vapor evaporator, Schlenk line.

Instruments: Scanning Electron Microscope (SEM) (JSM 6490 LA, JEOL), E-Graph (ATTA Bio-instrument, Azure C400), CD spectrophotometer (JASCO J-725), BIORAD Biologic Duoflow, Photomicroscopes equipped with temperature controlling unit, UV-visible spectrophotometer with digital data processing facilities, ABLE Jar Fermenter (BMJ-PI), Flow cytometer (Attune NXT), Inverted microscope (Leica DFC420 C), High-performance Liquid Chromatography (HPLC), IVIS Spectrum imager, NMR(VNMR500 & 400 MHz).

Cells Worked With:

Cancer cells: GBM6, GBM10, GBM12, GBM22, HF2303, HF3253, HF2427, HF2353, HF3016, U-87, U251, MBAMD-231, MBAMD-157, MBAMD-468, SUM149, SUM159, MIA, HN26 and HN48.
Recombinant protein expression and molecular biological experiments: HEK293, BL21 (DE3), JM109, NEB stable, DH5αZ1, RJY100.

Monbukagakusho (MEXT) Honors Scholarship offered by JASSO (Japan Student Services Organization)   October 2016 – March 2017
Monbukagakusho (MEXT) scholarship from the government of Japan to pursue doctoral degree   October 2013 – September 2016
Scholarship from Board of Intermediate and Secondary Education for excellent result in Secondary School Certificate Examination   July 2001 – Jun 2003
  • Biophysical Society of Japan [Membership no. 6741], Bangladesh Chemical Society [Membership no. LM1684]
8. Associate Professor, Department of Nutrition and Food Engineering, Daffodil International University, Dhaka, Bangladesh
7. Postdoctoral Research Fellow, Neurology Research, Henry Ford Hospital, Detroit, MI, USA
6. Postdoctoral Scholar, Biological Engineering, Tufts University, Medford, MA, USA
5. AIST-RA, Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Sapporo, Japan
4. Research Assistant
Laboratory of Biomolecular Adaptation Science, Graduate School of life Science, Hokkaido University, Sapporo, Japan
3. Graduate Student and AIST Trainee
Laboratory of Biomolecular Adaptation Science, Graduate School of life Science, Hokkaido University, Sapporo, Japan
2. Assistant Chemist
Bangladesh Chemical Industries Corporation (BCIC),  Bangladesh
1. Lecturer of Chemistry
St. Joseph Higher Secondary School, 97, Asad Avenue, Mohammadpur, Dhaka.